Advances in Clinical Chemistry, Vol. 32 by Herbert E. Spiegel

By Herbert E. Spiegel

Compliment for the sequence: "Its continuity of pertinence, excellence, and authority continues to be unbroken - a tribute to the skilful modifying and writing concerned. each proficient laboratory employees should have on hand a replica of this volume." --Clinical Chemistry For greater than thirty years, this serial has broadened the technical scope and multiplied the clinical base of medical chemistry. those volumes make clear the parts of molecular biology, informatics, and the tracking of physiological parameters in serious occasions as they pertain to scientific chemistry. each one quantity of Advances in medical Chemistry comprises an index, and every bankruptcy comprises references.

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The rapid reverse blot PCR-SSOP technique, when used with improved PCR-RFLP procedures relying upon the use of more group-specific amplifications and more restriction enzymes, is reported to improve the accuracy of typing (TI). Direct sequencing of PCR products from exon 2 subsequent to immobilization of the biotinylated PCR product to streptavidin-coated magnetic beads is an approach to typing class I1 genes using fluorescent primers and DNA polymerase (Fl). Thus, HLA typing for the purposes of organ transplantation has been facilitated by the use of molecular methods.

Cystic Fibrosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Duchenne and Becker Muscular Dystrophy . . . . . . . . . . . . . . . . . Lymphoproliferative Disorders . . . . . . . . . . . . . . . , . . . . . . . Chronic Myelogenous Leukemia and Acute Leukemias . . . . Human Papilloma Virus . . . . . . . . . . . , . . . . . . . , . . . . . . . Clinical Applications of Molecular Technologies , .

Thus, biotinylated chromosome-specific DNA probes directed to a-satellite DNA have been used to hybridize to the centromeric region of the specific chromosome under high-stringency conditions such as high temperature, low salt concentration, or high concentration of formamide. 0), and 10% dextran sulfate at a probe concentration of 1 nanogram (ng)/td of the hybridization mixture (Pl). The hybridized biotinylated probe can be detected microscopically either with fluoresceinated avidin or avidin conjugated with enzyme such as alkaline phosphatase.

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